Ho LH, Dorstyn L, Lambrusco, L, Read SH, Kumar S (2007) Caspase-2 is required for cell death induced by cytoskeletal disruption. Submitted.
Dorstyn L, Kumar S (2007) A biochemical analysis of the activation of the Drosophila caspase DRONC. Submitted.
Cakouros D, Mills K, Denton D, Daish T, Paterson A, Kumar S (2007) Hormone mediated histone arginine methylation in Drosophila is mediated by the enzyme dLKR/SDH. Submitted.
Lee I-H, Dinudom A, Sanchez-Perez A, Kumar S, Cook DI (2007) Akt mediates the effects of insulin on epithelial sodium channel by inhibiting Nedd4-2. J. Biol. Chem. 282: in press.
Sanchez-Perez A, Kumar S, Cook DI (2007) GRK2 interacts with and phosphorylates Nedd4 and Nedd4-2. Biochem. Biophys. Res. Commun. 359: 611-615.
Ekberg J, Schuetz F, Boase NA, Conroy S-J, Manning J, Kumar S, Poronnik P, Adams DJ (2007) Regulation of the voltage-gated K+ channels KCNQ2/3 and KCNQ3/5 by ubiquitination: Novel role for Nedd4-2. J. Biol. Chem. 282:12135-12142
Dibbens LM, Ekberg J, Taylor I, Hodgson BL, Conroy S-J, Lensink IL, Kumar S, Zielinski MA, Harkin LA, Sutherland GR, Adams DJ, Berkovic SF, Scheffer IE, Mulley JC, Poronnik P (2007) NEDD4-2 as a candidate susceptibility gene for epileptic photosensitivity. Genes Brain Behav. In press. (doi: 10.1111/j.1601-183X.2007.00305.x)
Doumanis J, Dorstyn L, Kumar S (2007) Molecular determinants of the subcellular localization of the Drosophila Bcl-2 homologues DEBCL and BUFFY. Cell Death Differ. 14: 907-915.
Sang Q, Kim MH, Kumar S, Bye N, Morganti-Kossman MC, Gunnersen J, Fuller S, Howitt J, Hyde L, Beissbarth T, Scott HS, Silke J, Tan SS (2006) Nedd4-WW domain-binding protein 5 (Ndfip1) is associated with neuronal survival after acute cortical brain injury. J. Neurosci. 26: 7234-7244.
Mills K, Daish, T, Harvey KF, Pfleger CM, Hariharan IK, Kumar S (2006) The Drosophila melanogaster Apaf-1 homologue ARK is required for most, but not all, programmed cell death. J. Cell Biol. 172: 809-815.
Rauh R, Dinudom A, Fotia AB, Paulides M, Kumar S, Korbmacher C, Cook DI (2006) Stimulation of the epithelial sodium channel (ENaC) by the serum- and glucocorticoid-inducible kinase (Sgk) involves the PY-motifs of the channel but is independent of sodium feedback inhibition. Pflügers Archiv-Eur. J. Physiol. 452: 290-299.
Fotia AB, Cook DI, Kumar S (2006) The ubiquitin-protein ligases Nedd4 and Nedd4-2 show similar ubiquitin-conjugating enzyme specificities. Int. J. Biochem. Cell Biol. 23: 472-479.
Umadevi N, Kumar S, Narayana N (2005) Crystallization and preliminary X-ray diffraction studies of the WW4 domain of the Nedd4-2 ubiquitin-protein ligase. Acta Crystallo. F61: 1084-1086.
Kilpatrick Z, Cakouros D, Daish TJ, Kumar S (2005) Ecdysone-mediated upregulation of the effector caspase DRICE is required for hormone-dependent apoptosis in Drosophila cells. J. Biol. Chem. 280: 11981- 11986.
Hryciw DH, Ekberg J, Lee A, Lensink IL, Kumar S, Guggino WB, Cook DI, Carol A. Pollock CA, Poronnik P (2004) Nedd4-2 functionally interacts with ClC-5: involvement in constitutive albumin endocytosis in proximal tubule cells. J. Biol. Chem. 279: 54996-55007.
Daish TJ, Mills K, Kumar S (2004) Drosophila caspase DRONC is required for specific developmental cell death pathways and stress-induced apoptosis. Dev. Cell 7: 909-915.
Chew SK, Akdemir F, Chen P, Lu, W-J, Mills K, Daish, T, Kumar S, Rodriguez A, Abrams JM (2004) The apical caspase, dronc, governs programmed and unprogrammed cell death in Drosophila. Dev. Cell 7: 897-907.
Dorstyn L, Mills K, Lazebnik Y, Kumar S (2004) The two cytochrome c species, DC3 and DC4, are not required for caspase activation and apoptosis in Drosophila cells. J. Cell Biol. 167: 405-410.
Baliga BC, Read SH, Kumar S (2004) The biochemical mechanism of caspase-2 activation. Cell Death Differ. 11: 1234-1241.
Dinudom A, Fotia A, Lefkowitz RJ, Young JA, Kumar S, Cook DI (2004) The kinase Grk2 regulates the Nedd4/Nedd4-2 dependent control of epithelial Na+ channels. Proc. Natl. Acad. Sci. USA. 101: 11886-11890.
Shearwin-Whyatt LM, Brown DL, Wylie FG, Stow JL, Kumar S (2004) N4WBP5A (Ndfip2), a Nedd4-interacting protein, localizes to multivesicular bodies and the Golgi, and has a potential role in protein trafficking. J. Cell Sci. 117: 3679-3689.
Fotia AB, Ekberg J, Cook DI, Adams DJ, Poronnik P, Kumar S (2004) Regulation of neuronal voltage-gated sodium channels by the ubiquitin-protein ligases Nedd4 and Nedd4-2. J. Biol. Chem. 279: 28930-28935
Murdaca J, Treins C, Monthouël-Kartmann MN, Pontier-Bres R, Kumar S, Van Obberghen E, Giorgetti-Peraldi S (2004) Grb10 prevents Nedd4-mediated VEGF receptor-2 degradation. J. Biol. Chem. 279: 26754-26761
Ekert PG, Read SH, Silke J, Marsden V, Kaufmann H, Hawkins CJ, Gerl R, Kumar S, Vaux DL (2004) Apaf-1, caspase-2 and caspase-9 accelerate apoptosis, but do not determine whether factor-deprived or drug-treated cells die. J. Cell Biol. 165: 835-842.
Cakouros D, Daish TJ, Kumar S (2004) Ecdysone receptor directly binds the promoter of the Drosophila caspase dronc regulating its expression in specific tissues. J. Cell Biol. 165: 631- 640.
Cakouros D, Daish TJ, Mills K, Kumar S (2004) An arginine-histone methyl transferase, CARMER, coordinates ecdysone-mediated apoptosis in Drosophila cells. J. Biol. Chem. 279:18467-18471.
Daish T, Cakouros D, Kumar S (2003) Distinct promoter regions regulate spatial and temporal expression of the Drosophila caspase dronc. Cell Death Differ. 10: 1348-1356.
Quinn L, Coomb M, Mills K, Daish T, Colussi P, Kumar S, Richardson H (2003) Buffy, a Drosophila Bcl-2 related protein, has anti-apoptotic and cell cycle inhibitory function. EMBO J. 22: 3568-3579.
Kim TW, Hung C-F, Ling M, Juang J, He L, Hardwick JM, Kumar S, Wu T-C (2003) Enhancing DNA vaccine potency by coadministration of DNA encoding antiapoptotic proteins. J. Clin. Invest. 112: 110-117.
Baliga BC, Colussi PA, Read SH, Dias MM, Jans DA, Kumar S (2003) Role of prodomain in importin-mediated nuclear localization and activation of caspase-2. J. Biol. Chem. 278: 4899-4905.
Full text published on-line on November 1, 2002 as 10.1096/fj.02-0497fje (FJ Express article).
Fotia A, Dinudom A, Shearwin KE, Koch J-P, Korbmacher C, Cook DI, Kumar S (2003) The role of individual Nedd4-2 (KIAA0439) WW domains in binding and regulating epithelial sodium channels. FASEB J. 17: 70-72.
Read SH, Baliga BB, Ekert P, Vaux DL, Kumar S (2002) A novel Apaf-1-independent putative caspase-2 activation complex. J. Cell Biol. 159: 739-745.
Konstas A-A, Shearwin-Whyatt LM, Fotia A, Degger B, Riccardi D, Cook DI, Korbmacher C, Kumar S (2002) Regulation of the epithelial sodium channel by N4WBP5A, a novel Nedd4/Nedd4-2-interacting protein. J. Biol. Chem. 277: 29406-29416.
Cakouros D, Daish T, Martin D, Baehrecke EH, Kumar S (2002) Ecdysone-induced expression of the caspase Dronc during hormone dependent programmed cell death in Drosophila is regulated by Broad-Complex. J. Cell Biol. 157: 985-995
O'Reilly LA, Ekert P, Harvey N, Marsden V, Cullen L, Vaux DL, Hacker G, Magnusson C, Pakusch M, Cecconi F, Strasser A, Huang DCS, Kumar S (2002) Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is mediated by Apaf-1 and caspase-9. Cell Death Diff. 9: 832-841.
Dorstyn L, Read S, Cakouros D, Huh JR, Hay BA, Kumar S (2002) The role of cytochrome c in caspase activation in Drosophila cells. J. Cell Biol. 156: 1089-1098.
Harvey KF, Shearwin-Whyatt LM, Fotia A, Parton RG, Kumar S (2002) N4WBP5, a potential target for ubiquitination by the Nedd4 family of proteins, is a novel Golgi-associated protein. J. Biol. Chem. 277: 9307-9317.
Shcherbik N, Kumar S, Haines DS (2002) Substrate proteolysis is inhibited by dominant-negative Nedd4 and Rsp5 mutants harboring alterations in WW domain 1. J. Cell Sci. 115: 1041-1048.
Harvey NL, Daish T, Mills K, Dorstyn L, Quinn LM, Read SH, Richardson H, Kumar S (2001) Characterization of the Drosophila caspase, DAMM. J. Biol. Chem. 276: 25342-25350.
Shearwin-Whyatt L, Baliga BC, Doumanis J, Kumar S (2001) Chimeric caspase molecules with potent cell killing activity in apoptosis resistant cells. Biochem. Biophys. Res. Commun. 282: 1114-1119.
Doumanis J, Quinn L, Richardson H, Kumar S (2001) STRICA, a novel Drosophila caspase with an unusual serine/threonine-rich prodomain, interacts with DIAP1 and DIAP2. Cell Death Diff. 8: 387-394.
Harvey KF, Dinudom A, Cook DI, Kumar S (2001) The Nedd4-like protein KIAA0439 is a potential regulator of the epithelial sodium channel. J. Biol. Chem. 276: 8597-8601.
Dinudom A, Harvey KF, Komwatana P, Jolliffe CN, Young JA, *Cook DI, *Kumar S (2001) The roles of the carboxyl termini of a-, b- and g-subunits of epithelial Na+ channels (ENaC) in regulating ENaC and in mediating its inhibition by cytosolic Na+. J. Biol. Chem. 276: 13744-13749. (*equal senior authors)
Quinn LM, Dorstyn L, Mills K, Colussi PA, Chen P, Coombe M, Abrams J, *Richardson H, *Kumar S (2000) An essential role for the caspase Dronc in developmentally programmed cell death in Drosophila. J. Biol Chem. 275: 40416-40424. (*equal senior authors)
Jolliffe CN, Harvey KF, Haines BP, Parasivam G, Kumar S (2000) Identification of multiple proteins expressed in murine embryos as binding partners for the WW domains of the ubiquitin-protein ligase Nedd4. Biochem. J. 351: 557-565.
Colussi PA, Quinn LM, Huang DCS, Coombe M, Read SH, Richardson H, Kumar S (2000) Debcl, a pro-apoptotic Bcl-2 homologue, is a component of the Drosophila cell death machinery. J. Cell Biol. 148: 703-714.
Shearwin-Whyatt LM, Harvey NL, Kumar S (2000) Subcellular localization and CARD-dependent oligomerization of the death adaptor RAIDD. Cell Death Diff. 7: 155-165.